窦敏敏;赵迪鹏;王鹤洁;郭田燕;李俊玲;李君;王聪慧;秦健;李颖靓;杜荣;

• 1:山西农业大学动物医学学院
• 2:山西农业大学实验教学中心

摘要(Abstract)：

为了研究MRFs家族中Myf5基因在不同发育时期绵羊胎儿骨骼肌中的表达规律及分子特性，实验采取妊娠30 d(E30)、50 d(E50)和70 d(E70)3个时期绵羊胎儿的半腱肌和背最长肌，用HE染色法观察了其显微结构，通过实时荧光定量PCR技术检测了Myf5基因的表达变化，同时运用生物信息学软件对绵羊Myf5蛋白的结构特征和理化特性及下游靶基因进行了预测分析。结果显示，E30～E70胎羊半腱肌和背最长肌的肌细胞数量和直径随胎龄逐渐增加，同一时期两者微观结构有所差异；Myf5 mRNA的表达量同样随胎龄而升高，但在同一时期2种组织中也存在差异。绵羊Myf5蛋白由255个氨基酸组成，分子式为C_(1209)H_(1901)N_(351)O_(397)S_(18)，定位于细胞核，主要由α螺旋和无规则卷曲组成，存在1个bHLH结构域，亲水性平均系数为-0.658，不稳定系数为72.99，等电点(PI)为5.72。绵羊Myf5调控的下游靶基因包括MyoD、MyoG和Myf6等；Myf5基因mRNA在胎羊半腱肌和背最长肌E30～E70时期的表达量均呈现上升趋势，但同一时期在背最长肌中的表达更高。本实验可为绵羊Myf5基因调控和作用机制的深入研究奠定基础。

关键词(KeyWords)： 绵羊胎儿;骨骼肌;Myf5基因;表达规律;生物信息学分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31872438);; 山西省基础研究计划(自由探索类)自然科学研究面上项目(20210302123394);; 山西省重点研发计划项目(201803D221022-4);; 山西省留学回国人员科技活动择优资助项目;; 山西省平台基地和人才专项优秀人才科技创新项目(201605D211028);; 山西农业大学中青年拔尖创新人才支持计划(BJRC201204);山西农业大学中青年学术带头人项目(XD201206);山西农业大学横向科技项目(2015HX12)

作者(Authors): 窦敏敏;赵迪鹏;王鹤洁;郭田燕;李俊玲;李君;王聪慧;秦健;李颖靓;杜荣;

DOI: 10.19556/j.0258-7033.20210928-02

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