李碧侠;赵芳;任守文;王学敏;方晓敏;付言峰;涂枫;王金玉;

• 1:扬州大学动物科学与技术学院
• 2:江苏省农业科学院畜牧研究所

摘要(Abstract)：

本研究旨在分析体外白藜芦醇对去乙酰化酶SIRT1表达量的影响,探讨去乙酰化酶SIRT1表达量与颗粒细胞凋亡间的相关性。采用qRT-PCR方法进行SIRT1 mRNA分析,Annexin V-FITC细胞凋亡检测试剂盒和流式细胞仪检测猪卵巢颗粒细胞凋亡率。结果表明:培养液中白黎芦醇浓度达到50μmol/L时,处理组颗粒细胞中SIRT1 mRNA表达量极显著高于空白对照组(P<0.01);与颗粒细胞中SIRT1表达量规律相同,随着白黎芦醇浓度的升高,颗粒细胞活力逐渐降低,凋亡率逐渐升高。综合分析,白藜芦醇可诱导猪卵巢颗粒细胞中去乙酰化酶SIRT1的表达,加速猪卵巢颗粒细胞凋亡,SIRT1表达与猪卵巢颗粒细胞凋亡存在一定相关性。

关键词(KeyWords)： 白黎芦醇;颗粒细胞;SIRT1;凋亡;猪

Abstract:

Keywords:

基金项目(Foundation): 江苏省自然科学基金(BK2010466);; 江苏省农业科技自主创新资金(CX(10)422);; 国家生猪现代产业技术体系南京综合试验站(CARS-36);; 江苏省农业种质资源库

作者(Authors): 李碧侠;赵芳;任守文;王学敏;方晓敏;付言峰;涂枫;王金玉;

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