益生菌发酵饲料对长白种公猪精液品质的影响
程泽超;韩培源;武丹;杨公社;胡建宏;李晓;
摘要(Abstract):
试验旨在研究益生菌发酵饲料对长白种公猪精液品质的影响。选取20头(150±20)日龄、体重(107.9±12.5)kg的成年长白公猪,采取单因素试验设计,随机分成5组,每组4头。对照组饲喂基础日粮,4个试验组分别以5%、10%、15%和20%发酵饲料替代基础日粮,50 d时采集血液,60 d时采集精液和粪便,测定血液生化、免疫、精液品质和粪便微生物相关指标。结果表明:与对照组相比,发酵饲料提高了公猪血清IgA、IgG和IgM的水平(P<0.05);5%、10%和15%发酵饲料替代组A级精子比例、精子活率、曲线速度、路径速度和摆动性(P<0.05)及精子顶体完整率均高于对照组(P<0.01);与对照组相比,10%、15%发酵饲料替代组精子质膜完整率均升高(P<0.01),发酵饲料均提高了公猪精浆果糖水平(P<0.05);粪便微生物测序结果显示,与对照组相比,15%发酵饲料替代组降低了粪便中厚壁菌门的丰度(P<0.05),提高了拟杆菌门的丰度(P<0.05);公猪粪便菌群组成与精液品质存在显著相关。在本试验条件下,10%和15%发酵饲料替代组精液品质更好。
关键词(KeyWords):
发酵饲料;公猪;精液品质;粪菌菌群
基金项目(Foundation): 陕西省重点研发计划(2019NY-078);; 陕西省农业专项基金(NYKJ-2018-YL01)
作者(Authors):
程泽超;韩培源;武丹;杨公社;胡建宏;李晓;
DOI: 10.19556/j.0258-7033.20210625-03
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