中国畜牧杂志

2022, v.58(07) 122-133

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苏博美利奴羊毛囊发育不同时期ncRNA调控网络的构建
Construction of ncRNA Network Related to Hair Follicle Development in Subo Merino Sheep

张小伟;何军敏;徐新明;田月珍;关鸣轩;张漫;付雪峰;哈尼克孜·吐拉甫;黄锡霞;田可川;
ZHANG Xiaowei;HE Junmin;XU Xinming;TIAN Yuezhen;GUAN Mingxuan;ZHANG Man;FU Xuefeng;Hanicki Turap;HUANG Xixia;TIAN Kechuan;College of Animal Science, Xinjiang Agricultural University;Institute of Animal Science, Xinjiang Academy of Animal Science;Institure of Animal Science and Veterinary Medicine Shangdong Academy of Agricultural Sciences;

摘要(Abstract):

为探索苏博美利奴羊毛囊发育相关ncRNA分子调控网络,本研究通过挖掘苏博美利奴羊皮肤组织样本不同时期转录组数据,利用生物信息学方法构建lncRNA-miRNA-mRNA分子调控网络。结果表明:不同时期mRNA与lncRNA及miRNA具有复杂的网络连接。通过GO富集分析发现靶基因主要参与细胞增殖调控、凋亡过程及毛囊形态发生等生物学过程;参与细胞外空间、细胞外泌体、转录因子复合体、质膜的整体成分等细胞组分;参与转录因子活性、结构分子活性、细胞外基质结构组成等分子功能。通过KEGG通路分析发现差异的靶基因主要参与TGF-beta信号通路、癌症途径、MAPK信号通路、PI3K-Akt信号通路、细胞因子与细胞因子受体的相互作用等多种途径。筛选寻找调控网络中的关键基因,发现G2 vs.G1组中miR-133、miR-433-5p的靶基因TGFβ2和NOTCH1参与毛囊形态发生(GO:0031069)的生物学过程,因此TGFβ2和NOTCH1为毛囊发育的关键基因。通过构建苏博美利奴羊毛囊发育相关ncRNA分子调控网络,解析毛囊发生发育的分子调控机制,从转录组学层面对毛囊生长发育进行探讨,能够对苏博美利奴羊毛囊发育的分子作用机制进行系统性挖掘。
To explore the ncRNA molecular regulatory network related to the development of Subo merino wool follicles, this study used bioinformatics methods to construct lncRNA-miRNA-mRNA molecular regulatory network by mining Subo merino sheep skin tissue samples at different periods of transcriptome data, and the results showed: mRNA and lncRNA and miRNA have complex network connections in different periods. Through GO enrichment analysis, it is found that target genes are mainly involved in biological processes such as negative regulation of cell proliferation、negative regulation of apoptotic process, and hair follicle morphogenesis; they are involved in cell components such as extracellular space, extracellular exosome, transcription factor complex and integral component of plasma membrane; Participate in molecular functions such as transcriptional activator activity, structural molecular activity, and extracellular matrix structural constituent. Through KEGG pathway analysis, it is found that the different target genes are mainly involved in the TGF-beta signaling pathway, Pathways in cancer, MAPK signaling pathway, PI3 K-Akt signaling pathway, and Cytokine-cytokine receptor interaction. Screening for key genes in the regulatory network, it was found that the target genes TGFβ2 and NOTCH1 of miR-133 and miR-433-5 p in the G2 vs.G1 group are involved in the biological process of hair follicle morphogenesis(GO:0031069), so TGFβ2 and NOTCH1 are hair follicles Key genes for development. By constructing the ncRNA molecular regulation network related to the development of Subo merino wool follicles, analyzing the molecular regulation mechanism of hair follicle development, discussing the growth and development of hair follicles from the transcriptomics level, and systematically studying the molecular mechanism of subo merino wool follicle development Sexual mining.

关键词(KeyWords): miRNA;lncRNA;mRNA;毛囊发育;调控网络;苏博美利奴羊
miRNA;LncRNA;mRNA;Development of wool follicles;Regulatory network;Subo Merino

Abstract:

Keywords:

基金项目(Foundation): 国家现代农业产业技术体系建设项目(CARS-40);; 国家自然科学基金(31760655);; 新疆维吾尔自治区重点实验室开放项目(2016D03017)

作者(Authors): 张小伟;何军敏;徐新明;田月珍;关鸣轩;张漫;付雪峰;哈尼克孜·吐拉甫;黄锡霞;田可川;
ZHANG Xiaowei;HE Junmin;XU Xinming;TIAN Yuezhen;GUAN Mingxuan;ZHANG Man;FU Xuefeng;Hanicki Turap;HUANG Xixia;TIAN Kechuan;College of Animal Science, Xinjiang Agricultural University;Institute of Animal Science, Xinjiang Academy of Animal Science;Institure of Animal Science and Veterinary Medicine Shangdong Academy of Agricultural Sciences;

DOI: 10.19556/j.0258-7033.20210203-01

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