中国畜牧杂志

2022, v.58(07) 199-203

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甘氨酸对脂多糖应激断奶仔猪肠黏膜屏障的调控作用
Regulation of Glycine on Intestinal Mucosal Barrier Induced in Weaned Pigs Challenged Lipopolysaccharide

王慧;郭俊杰;李先根;任效瑞;刘玉兰;杨彩梅;朱惠玲;
WANG Hui;GUO Junjie;LI Xiangen;REN Xiaorui;LIU Yulan;YANG Caimei;ZHU Huiling;Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University;Zhejiang Huijia Biotechnology Co., Ltd.;

摘要(Abstract):

本试验旨在研究日粮中添加甘氨酸(Gly)对脂多糖(LPS)应激断奶仔猪肠黏膜屏障损伤的保护作用。选用24头21日龄断奶仔猪,随机分为4组,即对照组(基础日粮)、LPS组(基础日粮+LPS)、1.0%Gly组(基础日粮+1.0%Gly+LPS)、2.0%Gly组(基础日粮+2.0%Gly+LPS)。预饲期7 d,正试期28 d,在试验第28天,LPS组、1.0%Gly组和2.0%Gly组仔猪腹腔注射LPS(100μg/kg BW),对照组注射无菌生理盐水。注射4 h后,仔猪屠宰,取脾脏、肝脏、肠系膜淋巴结及空肠、回肠样品待测。结果表明:(1)各处理组的平均日增重、平均日采食量及耗料增重比无显著差异。(2)与对照组相比,LPS应激升高了回肠丙二醛(MDA)水平(P<0.05),降低空肠、回肠谷胱甘肽(GSH)水平(P<0.05);与LPS组相比,日粮中添加Gly降低回肠MDA水平、升高GSH水平(P<0.05)。(3)与对照组相比,LPS应激增加肝脏移位细菌数(P<0.05);与LPS组相比,日粮中添加Gly降低肝脏移位细菌数(P<0.05)。(4)与对照组相比,LPS应激降低空肠绒毛高度/隐窝深度比值(P<0.05),且有降低空肠绒毛高度趋势(P<0.1);与LPS组相比,日粮中添加Gly对空肠、回肠的绒毛高度、隐窝深度及绒毛高度/隐窝深度比值没有显著影响。(5)与对照组相比,LPS应激增加回肠肥大细胞密度(P<0.05);与LPS组相比,日粮中添加Gly降低空肠肥大细胞密度(P<0.05)。(6)与对照组相比,LPS应激升高空肠、回肠CRF(P<0.05)、CRFR1(P<0.001)和GR(P<0.05)的mRNA相对表达量;与LPS相比,日粮添加Gly降低了空肠、回肠CRFR1(P<0.05)及空肠GR(P<0.001)的mRNA相对表达量。结果提示,日粮添加Gly可缓解LPS应激导致的肠黏膜屏障功能障碍。
The aim of this study was to investigate the protective effect of glycine(Gly) on injury of intestinal mucosal barrier induced by lipopolysccharide(LPS) in weaned pigs. Twenty-four pigs weaned at 21 d of age were randomly divided into four groups: control group(basal diet), LPS group(basal diet + LPS), 1.0% Gly group(basal diet + 1.0% Gly + LPS), and 2.0%Gly group(basal diet + 2.0% Gly + LPS). Pigs were acclimated for 7 days to experimental conditions and fed control diet.The experiment lasted for 28 days. On d 28 of the trial, pigs were injected intraperitoneally with saline or LPS. After 4 hours of LPS or saline injection, pigs were slaughtered and spleen, mesenteric lymph, liver and intestine were collected. The results showed that:(1) There was no difference in average daily gain(ADG), average daily feed intake(ADFI) and feed/gain(F/G)among four treatments(P>0.05).(2) Compared with control group, LPS challenge increased the level of MDA in ileum(P<0.05),and decreased the level of GSH in jejunum and ileum(P<0.05). Compared with LPS group, Gly supplementation decreased the level of MDA and increased the level of GSH in ileum(P<0.05).(3) Compared with control group, LPS challenge increased bacterial translocation incidence and translocation microorganisms in the spleen(P<0.05). Compared with LPS group, Gly supplementation reduced translocation microorganisms in the liver(P<0.05).(4) Compared with the control group, LPS challenge decreased jejunal villus height/crypt depth ratio(P<0.05), and tended to decrease jejunal villus height(P<0.1). Compared with LPS group, Gly supplementation had no effects on villus height, crypt depth and villus height/crypt depth ratio in jejunum and ileum(P>0.05).(5) Compared with control group, LPS challenge increased mast cell density in ileum(P<0.05). Compared with LPS group, Gly supplementation decreased the density of jejunal mast cells(P<0.05).(6) Compared with control group,LPS challenge increased the mRNA expression of CRF(P<0.05), CRFR1(P<0.001), GR(P<0.05) in jejunum and ileum.Compared with LPS group, Gly supplementation decreased the mRNA expression of CRFR1 in jejunum and ileum(P<0.05)and GR(P<0.001) in jejunum. These results indicated that Gly alleviated the intestinal barrier dysfunction induced by LPS.

关键词(KeyWords): 甘氨酸;脂多糖;肠黏膜屏障;断奶仔猪
Glycine;Lipopolysaccharide;Intestinal mucosal barrier;Weaned piglets

Abstract:

Keywords:

基金项目(Foundation): 湖北省自然科学基金创新群体项目(2019CFA015);湖北省自然科学基金(2019CFB831);; 浙江省领军型创新创业团队项目(2020R01015);; 动物营养与饲料科学湖北省重点实验室开放课题(201901)

作者(Authors): 王慧;郭俊杰;李先根;任效瑞;刘玉兰;杨彩梅;朱惠玲;
WANG Hui;GUO Junjie;LI Xiangen;REN Xiaorui;LIU Yulan;YANG Caimei;ZHU Huiling;Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University;Zhejiang Huijia Biotechnology Co., Ltd.;

DOI: 10.19556/j.0258-7033.20210723-05

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