中国畜牧杂志

2020, v.56(02) 53-58

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绵羊主效基因FecB的检测与肉用多羔核心群的建立
The Detection of Major Gene FecB in Sheep and Establishment of Ovine Prolific and Mutton Core Herds

张壮彪;刘秋月;狄冉;胡文萍;王翔宇;贺小云;马琳;储明星;
ZHANG Zhuangbiao;LIU Qiuyue;DI Ran;HU Wenping;WANG Xiangyu;HE Xiaoyun;MA Lin;CHU Mingxing;Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences;

摘要(Abstract):

基于前期本实验室建立的绵羊多羔性状主效基因FecB高通量检测技术(TaqMan探针法),对10个绵羊群体的16 460只绵羊进行了多羔性状主效基因FecB的检测,结果发现小尾寒羊、湖羊以及滩羊的B等位基因频率与前人实验结果一致,有些群体经过长期选择已处于Hardy-Weinberg不平衡状态(P<0.05);本课题组与多个企业经过长期努力已建立了多个肉用多羔绵羊核心群,通过比较发现BB型绵羊产羔数极显著高于B+型,B+型极显著高于++型,且高繁殖力核心群3种FecB基因型小尾寒羊产羔数高于普通小尾寒羊群体。绵羊多羔性状主效基因FecB的检测和肉用多羔绵羊核心群的建立为绵羊群体改良提供了支撑。
The TaqMan probe method previously built by our laboratory was applied to detect the major gene FecB in 16460 sheep distributed in 10 populations. The results suggested that the B allele frequency in sheep populations containing Small Tail Han sheep, Hu sheep and Tan sheep was compatible to previous studies, however, several populations including Bamei mutton sheep, Luzhong mutton sheep had been under the state of Hardy-Weinberg disequilibrium(P<0.05) since long-term selection. Meanwhile, several prolific core herds of mutton sheep were also established through cooperating with farming enterprises. The statistical results showed that the litter size of sheep populations with BB genotype was significantly higher than that with B+, and litter size of B+ genotype was significantly higher than that of ++, in addition, the litter size of the three FecB genotypes in the prolific core herd of Small Tail Han sheep was higher than that in the common herd. The detection of FecB and establishment of prolific core herds of mutton sheep provided a support for improvement of sheep with relatively low fecundity.

关键词(KeyWords): 绵羊;FecB基因;TaqMan探针法;等位基因频率;高繁殖力核心群
Sheep;FecB gene;TaqMan probe method;Allele frequency;Prolific core herds

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31772580);; 国家肉羊产业技术体系专项(CARS-38);; 中国农业科学院科技创新工程(ASTIPIAS13);; 中央级公益性科研院所基本科研业务费专项(Y2017JC24);; 农业科研杰出人才及其创新团队项目(农办人[2015]62号);; 国家万人计划科技创新领军人才项目(W02020274);; 天津市农业科技成果转化与推广项目(201704020)

作者(Authors): 张壮彪;刘秋月;狄冉;胡文萍;王翔宇;贺小云;马琳;储明星;
ZHANG Zhuangbiao;LIU Qiuyue;DI Ran;HU Wenping;WANG Xiangyu;HE Xiaoyun;MA Lin;CHU Mingxing;Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences;

DOI: 10.19556/j.0258-7033.20190108-05

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