腾瑶瑶;李世杰;马兴红;

• 1:东北农业大学生命科学学院

摘要(Abstract)：

SerpinB6b是丝氨酸蛋白酶抑制剂亚家族B成员之一。本实验旨在利用荧光定量PCR、原位杂交、免疫组织化学与蛋白质印迹分析等实验技术,研究SerpinB6b mRNA和蛋白在大鼠早期妊娠1～9 d、人工诱导蜕膜化及体外诱导蜕膜化模型子宫中的表达规律。结果显示:SerpinB6b mRNA和蛋白在大鼠早期妊娠1～5 d腔上皮、腺上皮有微弱表达;在6～9 d,表达主要集中在胚胎和蜕膜区,且随蜕膜化程度增加,表达量逐渐增强,且在第8天达到最高;在大鼠人工诱导蜕膜化模型中,SerpinB6b在蜕膜区的表达均显著高于非蜕膜区;在大鼠子宫内膜基质细胞体外诱导蜕膜化模型中,SerpinB6b随着诱导蜕膜化天数的增加呈上升趋势。以上体内和体外实验结果表明,SerpinB6b在大鼠子宫中的表达具有着床相关特异性,SerpinB6b参与大鼠子宫蜕膜化过程。

关键词(KeyWords)： SerpinB6b;大鼠;子宫;蜕膜化

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31271601、31571553);; 黑龙江省教育厅海外学人科研资助项目(1253HQ016)

作者(Authors): 腾瑶瑶;李世杰;马兴红;

DOI: 10.19556/j.0258-7033.20190428-02

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