陆奇;韩修远;赵亮;刘爽;亓美玉;姚玉昌;

• 1:黑龙江普通高等学校动物遗传育种与繁殖重点实验室
• 2:东北农业大学动物科学技术学院
• 3:黑龙江省农业科学院畜牧研究所

摘要(Abstract)：

精子在生殖道内运行过程中易受到革兰氏阴性菌侵染，导致精子活力下降，进而造成受精能力降低。本研究采用上浮法获得高活力、高纯度的绵羊精子样品(n=8)，以LPS(1μg/mL)作为模拟物处理精子，阐明其对精子活力、线粒体结构及功能的影响，并揭示TLR4基因在此过程中的介导作用。结果表明：LPS处理后6 h时精子活力下降(P<0.001),TLR4基因表达上调(P<0.001)，同时其表达位置由头部向尾部扩展；过表达TLR4基因对精子活力无显著影响，而利用TAK-242抑制TLR4信号通路缓解了LPS诱发的精子活力下降(P<0.001)。LPS处理后精子线粒体出现不规则排列、膜间隙肿胀、定向障碍和大量的液泡结构，同时膜电位水平降低和ATP减少(P<0.001)；过表达TLR4基因对线粒体膜电位和ATP的产生无显著影响，而TAK-242处理改善了LPS诱发的线粒体膜电位水平降低(P<0.05)和ATP产生减少(P<0.001)。本研究表明革兰氏阴性菌细胞壁成分—LPS可诱发绵羊精子活力降低、线粒体结构及功能受损，TLR4基因在此过程中扮演着重要的介导作用。

关键词(KeyWords)： 绵羊;脂多糖;精子活力;线粒体;TLR4基因

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省博士后科研启动资助金项目(LBH-Q18131);; 东北农业大学“学术骨干”基金资助项目(19XG12)

作者(Authors): 陆奇;韩修远;赵亮;刘爽;亓美玉;姚玉昌;

DOI: 10.19556/j.0258-7033.20210531-03

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