中国畜牧杂志

2021, v.57(11) 45-50

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

基因编辑技术在家畜育种中的研究进展
Research Progress of Gene Editing Technology in Livestock Breeding

邹菊红;邹剑伟;申玉建;冯雪萍;连子童;徐建建;宋颖;胡艳;张瑜;黄艳娜;蒋钦杨;
ZOU Juhong;ZOU Jianwei;SHEN Yujian;FENG Xueping;LIAN Zitong;XU Jianjian;SONG Ying;HU yan;ZHANG Yu;HUANG Yannan;JIANG Qinyang;College of Animal Science and Technology,Guangxi University;

摘要(Abstract):

基因编辑技术可以从分子水平上对某个基因进行定点修饰,从而带给生物体长久的可遗传的变异,被广泛运用于生命科学研究以及临床应用。目前基因编辑的DNA核酸酶主要有锌指核酸酶(Zinc Finger Nucleases,ZFNs)、转录激活因子样效应物核酶(Transcription Activator-Like Effector Nucleases,TALENs)和成簇规则间隔的短回文重复序列/Cas9系统(Clustered Regularly Interspaced Short Palindromic Repeats,CRISPRs/Cas9)。这些DNA核酸酶通过在预定的基因组位点诱导双链断裂来提高DNA突变率而介导靶向的遗传改变,可用来改良动物性状,在家畜育种中发挥极大作用。本文综述了几种基因编辑工具的基因结构、作用原理、几种技术之间的差异及在家畜育种中的应用情况,为基因编辑技术在家畜育种中的研究提供参考。
Gene editing technology can modify a gene at the molecular level, thus bringing long-term heritable variation to organisms, which is widely used in life science research and clinical applications. At present, the main DNA nucleases edited by genes are zinc flnger nuclease(zinc flnger nucleases, ZFNs), transcriptional activator-like ribozymes(transcription activator-like effector nucleases TALENs) and clusters of regularly spaced short palindromic repeats/Cas9 system(clustered regularly interspaced short palindromic repeats, CRISPRs/Cas9). These DNA nucleases mediate targeted genetic changes by inducing double-strand breaks at predetermined genome sites to increase the rate of DNA mutation, which can be used to improve animal traits and play an important role in livestock breeding. This paper summarizes the gene structure and function principle of several gene editing tools, analyzes the differences among several techniques, and focuses on their application in livestock breeding, so as to provide reference for the research of gene editing technology in livestock breeding.

关键词(KeyWords): 基因编辑技术;家畜;ZFNs;TALENs;CRISPR/Cas9系统
Gene editing technology;Livestock;ZFNs;TALENs;CRISPR/cas9 system

Abstract:

Keywords:

基金项目(Foundation): 广西重点研发计划项目(桂科AB18221067);; 广西牛羊产业创新团队(nycytxgxcxtd-2021-09);; 广西创新驱动发展专项(桂科AA17204052);; 崇左市科技计划项目(崇科FC2018006)

作者(Authors): 邹菊红;邹剑伟;申玉建;冯雪萍;连子童;徐建建;宋颖;胡艳;张瑜;黄艳娜;蒋钦杨;
ZOU Juhong;ZOU Jianwei;SHEN Yujian;FENG Xueping;LIAN Zitong;XU Jianjian;SONG Ying;HU yan;ZHANG Yu;HUANG Yannan;JIANG Qinyang;College of Animal Science and Technology,Guangxi University;

DOI: 10.19556/j.0258-7033.20201027-04

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享