中国畜牧杂志

2020, v.56(02) 106-110

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白菜尾菜与稻草秸秆混合青贮饲料对瘤胃微生物体外发酵的影响
Effects of Mixed Silage of Rice Straw and Chinese Cabbage Waste on Rumen Microbial Fermentation in vitro

常荣鑫;戚如鑫;史剑云;张振斌;王梦芝;
CHANG Rongxin;QI Ruxin;SHI Jianyun;ZHANG Zhenbin;WANG Mengzhi;College of Animal Science and Technology, Yangzhou University;

摘要(Abstract):

本试验旨在研究不同处理的白菜尾菜与稻草秸秆混合青贮饲料对瘤胃微生物体外发酵的影响。试验底物为4种不同处理的青贮饲料,青贮45 d:对照组(秸秆:尾菜为10:0;无添加剂),1组、2组、3组的秸秆∶尾菜均为4:6,但添加剂分别为植物乳杆菌0.030 g/kg和纤维素酶0.200 g/kg、植物乳杆菌0.035 g/kg和纤维素酶0.250 g/kg、植物乳杆菌0.040 g/kg和纤维素酶0.300 g/kg,进行体外瘤胃发酵36 h,测定pH、氨氮浓度、微生物蛋白浓度等发酵参数。结果表明:培养期间,各组的pH在6.77~6.94、氨氮浓度在20.09~44.39 mg/100mL;在12 h、36 h时,2组的微生物蛋白浓度显著高于对照组和1组;在36 h时,2组和3组的原虫密度显著高于对照组,且酸性洗涤纤维降解率也较高。综上,2组和3组都能在一定程度上改善瘤胃发酵性能,其中,以第2组采用秸秆:尾菜为4:6、植物乳杆菌0.035 g/kg和纤维素酶0.250 g/kg的组合处理青贮饲料较为适宜。
The purpose of this experiment was to investigate the effects of different treatments of rice straw and chinese cabbage waste mixed silage on in vitro fermentation of rumen microorganisms. The experimental substrate was 4 different silages with a silage time of 45 days: the control group(rice straw:chinese cabbage waste = 10:0; no additive), and the ratio of rice straw:chinese cabbage waste in group 1, group 2 and group 3 were 4:6, but the additive amount were Lactobacillus plantarum 0.030 g/kg and cellulase 0.200 g/kg, Lactobacillus plantarum 0.035 g/kg and cellulase 0.250 g/kg, Lactobacillus plantarum 0.040 g/kg and cellulase 0.300 g/kg, in vitro rumen fermentation for 36 h, determination of pH, ammonia nitrogen concentration, microbial protein concentration and other fermentation parameters. The results showed that the pH of each group was 6.77~6.94 and the ammonia nitrogen concentration was 20.09~44.39 mg/100 mL during fermentation. When 12 h and 36 h, the microbial protein concentrations of group 2 was higher than that of control group and group 1(P<0.05). When 36 h, the density of protozoa in group 2 and group 3 was significantly higher than that in control group(P<0.05), and the degradation rate of acid detergent fiber(ADF) in these two groups was also higher than other groups. In summary, both group 2 and group 3 all can improve rumen fermentation performance to some extent. Among group 2 and group 3, group 2 mixed silage of rice straw:chinese cabbage waste=4:6, Lactobacillus plantarum 0.035 g/kg and cellulase 0.250 g/kg was better than others.

关键词(KeyWords): 白菜尾菜;稻草秸秆;混合青贮;瘤胃发酵;绵羊
Chinese cabbage waste;Rice straw;Mixed silage;Rumen fermentation;Sheep

Abstract:

Keywords:

基金项目(Foundation): “十三五”国家重点研发计划项目(2018YFD0502100、2017YFD0800200);; 江苏省高校优势学科建设工程资助项目(PAPD)

作者(Authors): 常荣鑫;戚如鑫;史剑云;张振斌;王梦芝;
CHANG Rongxin;QI Ruxin;SHI Jianyun;ZHANG Zhenbin;WANG Mengzhi;College of Animal Science and Technology, Yangzhou University;

DOI: 10.19556/j.0258-7033.20190502-01

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