中国畜牧杂志

2020, v.56(02) 84-87

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lncRNA-47491、lncRNA-49770和lncRNA-51636在发生炎症反应仔猪肝脏和脾脏中的表达分析
Expression Analysis of lncRNA-47491, lncRNA-49770 and lncRNA-51636 in Liver and Spleen Tissue of Piglets with Inflammatory Response

徐鑫;黄兴法;王秀英;汪文俊;朱惠玲;刘玉兰;张晶;
XU Xin;HUANG Xingfa;WANG Xiuying;WANG Wenjun;ZHU Huiling;LIU Yulan;ZHANG Jing;Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University;Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities;

摘要(Abstract):

本试验旨在研究脂多糖(LPS)诱导仔猪发生炎症反应时肝脏和脾脏炎性细胞因子和lncRNA-47491、lncRNA-49770、lncRNA-51636表达的变化。选取8头21日龄、体重(7.15±0.42)kg的杜×长×大三元杂交断奶仔猪,随机分为对照组、LPS组,每个处理4个重复,每个重复1头猪。预试14 d后,LPS组和对照组腹腔分别注射100μg/kg体重的LPS、生理盐水,4 h后屠宰并取肝脏和脾脏组织样待测。结果表明:与对照组相比,LPS刺激使肝脏和脾脏中炎性细胞因子的mRNA表达量显著上调;LPS诱导的炎症反应中lncRNA-47491、lncRNA-49770和lncRNA-51636的表达量均显著上调;对lncRNA调控的顺式靶标基因的预测结果显示,lncRNA-47491和lncRNA-51636的靶基因有TMEM235、PGS1、TK1等,lncRNA-49770的靶基因有SRRM2、CLDN6、TNFRSF12A等,且这些基因均与炎症相关。综上,lncRNA-47491、lncRNA-49770及lncRNA-51636可能参与了机体的炎症反应,且靶标基因的预测提示其可能发挥不同的调控作用。
The purpose of this study was to investigate the changes in the expression of inflammatory cytokines and lncRNA-47491, lncRNA-49770, and lncRNA-51636 in liver and spleen induced by lipopolysaccharide(LPS) in piglets. A total of 8 weaned piglets(Duroc × Landrace × Yorkshine; weaned at 21 d of age;(7.15±0.42) kg initial body weight) were randomly divided into two groups(n=4), including control group and LPS group. After 14 days of pretest, the piglets in LPS group were injected with 100 μg/kg BW LPS and the piglets in control group were injected with an equivalent amount of sterile physiological saline solution. All piglets were slaughtered at 4 h post injections and tissue samples of liver and spleen were collected for analysis. The results showed that in liver and spleen tissues, LPS significantly increased the expression of inflammatory cytokines and lncRNA(lncRNA-47491, lncRNA-49770 and lncRNA-51636). Target gene prediction results show that the potential target genes of lncRNA-47491 and lncRNA-51636 including TMEM235, PGS1, TK1, target genes of lncRNA-49770 including SRRM2, CLDN6, TNFRSF12 A, and these genes are all associated with inflammation. Therefore, lncRNA-47491, lncRNA-49770 and lncRNA-51636 may be involved in the inflammatory response, and the prediction of these target genes revealed that they may play different regulatory roles.

关键词(KeyWords): lncRNA;脂多糖;肝脏;脾脏;断奶仔猪
lncRNA;Lipopolysaccharide;Liver;Spleen;Weaned piglets

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31702203);; 湖北省教育厅优秀中青年科技创新团队项目(T201508)

作者(Authors): 徐鑫;黄兴法;王秀英;汪文俊;朱惠玲;刘玉兰;张晶;
XU Xin;HUANG Xingfa;WANG Xiuying;WANG Wenjun;ZHU Huiling;LIU Yulan;ZHANG Jing;Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University;Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities;

DOI: 10.19556/j.0258-7033.20190704-03

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