程鹏;张静博;赵龙妹;曹平华;马彦博;李旺;
CHENG Peng;ZHANG Jingbo;ZHAO Longmei;CAO Pinghua;MA Yanbo;LI Wang;College of Animal Science and Technology,Henan University of Science and Technology;

• 1:河南科技大学动物科技学院

摘要(Abstract)：

试验旨在探讨不同乳酸菌(Lacticacidbacteria,LAB)饲喂方式对肉鸡生长性能、免疫器官与免疫因子的影响。将192只1日龄爱拔益加(AA)肉仔鸡(公母各半)，随机分为4组，每组6个重复，每个重复8只鸡。对照组饲喂基础日粮，拌料组在饲料中添加乳酸菌菌液，灌服组口腔灌服乳酸菌菌液，饮水组饮水中添加乳酸菌菌液，即自制乳酸菌菌液以不同方式等量饲喂给肉鸡，以保证每鸡每日摄取乳酸菌活菌数为8×108CFU。试验期为42 d。结果表明：1～21日龄，拌料组、饮水组耗料增重比低于对照组、灌服组(P<0.05);22～42日龄，不同饲喂方式均提高了平均日增重，降低了耗料增重比(P<0.05)，但不同饲喂方式间无显著差异；1～21日龄，饮水组、拌料组肉鸡胸腺指数高于对照组、灌服组(P<0.05),22～42日龄，灌服组胸腺指数高于对照组、拌料组(P<0.05)，与饮水组无显著差异；21日龄、42日龄，饮水组IgA含量高于其他处理组(P<0.05)，试验组的其余免疫因子含量均较对照组有不同程度提升，但不同饲喂方式间无显著差异。综上，拌料中添加乳酸菌菌液可提高1～21日龄胸腺指数，降低耗料增重比；灌服方式可提高22～42日龄胸腺指数；饮水饲喂乳酸菌可降低1～21日龄耗料增重比，稳定提高胸腺指数及IgA含量，生产中建议以饮水方式饲喂乳酸菌菌液。
The purpose of this experiment was to investigate the effects of different feeding methods of lactic acid bacteria on growth performance,immune organs and immune factors of broilers.A total of 192 1-day-old AA broilers(half male and half female) were randomly divided into 4 groups with 6 replicates in each group and 8 chickens in each replicate.The control group was fed the basic diet,the mixing group was fed with lactic acid bacteria solution in the feed,the oral cavity of the gavage group was fed with lactic acid bacteria solution,and the drinking water group was fed with lactic acid bacteria solution in water,that is,the self-made lactic acid bacteria solution was fed to broilers in equal quantities in different ways,so as to ensure that the daily intake of live lactic acid bacteria per chicken was 8×10⁸ CFU.The test period was 42 days.The results showed that the ratio of material consumption and weight gain in the mixed group and drinking group was lower than that in control group and gavage group at the age of 1～21 days(P<0.05);At 22 ～ 42 days of age,different feeding methods increased the average daily gain and reduced the feed consumption weight gain ratio(P<0.05);At the age of 1～21 days,the thymus index of broilers in drinking group and mixing group was higher than that in control group and gavage group(P<0.05),and at the age of 22～42 days,the thymus index of gavage group was higher than that in control group and mixing group(P<0.05);At 21 and 42 days of age,the content of IgA in drinking water group was higher than that in other groups(P<0.05).The content of other immune factors in the experimental group was higher than that in control group,but there was no significant difference between different feeding methods.In conclusion,adding lactic acid bacteria solution to the mixture can improve the thymus index at the age of 1～21 days and reduce the material consumption and weight gain ratio;The thymus index of 22～42 days old could be increased by oral administration;Feeding lactic acid bacteria with drinking water can reduce the feed consumption and weight gain ratio from 1 to 21 days old,and stably improve the thymus index and IgA content.It is suggested to feed lactic acid bacteria solution with drinking water in production.

关键词(KeyWords)： 乳酸菌;饲喂方式;肉鸡;生产性能;免疫机能
Lactic acid bacteria;Feeding mode;Broiler;Production performance;Immune function

Abstract:

Keywords:

基金项目(Foundation): 国家科技部专项“国际间重点联合项目”(2017YF E0129900);; 河南省重点攻关项目(212102110157、212102110174)

作者(Authors): 程鹏;张静博;赵龙妹;曹平华;马彦博;李旺;
CHENG Peng;ZHANG Jingbo;ZHAO Longmei;CAO Pinghua;MA Yanbo;LI Wang;College of Animal Science and Technology,Henan University of Science and Technology;

DOI: 10.19556/j.0258-7033.20210629-04

参考文献(References)：

• [1]陈琼.中国肉鸡生产的成本收益与效率研究[D].北京:中国农业科学院,2013:66.
• [2]Liao X D,Ma G,Cai J.et al.Effects of Clostridium butyricum on growth performance,antioxidation,and immune function of brilers[J].Poult Sci,2015,94(4):662-667.
• [3]Yang H,Paruch L,Chen X J,et al.Antibiotic application and resistance in swine production in china:current situation and future perspectives[J].Front Vet Sci,2019,6:136.
• [4]吴宇薇,杨海明,张泉.饲粮添加益生菌对肉鸡免疫机能的影响[J].中国家禽,2019,41(12):43-46.
• [5]Horackova S,Vesela K,Klojdova I,et al.Bile salt hydrolase activity,growth characteristics and surface properties in Lactobacillus acidophilus[J].Eur Food Res Technol,2020,246:1627-1636.
• [6]Park Y H,Hamidon F,Rajangan C,et al.Application of probiotics for the production of safe and high-quality poultry meat[J].Korean J Food Sci Anim Res,2016,36(5):567-576.
• [7]张炎达,潘慧青,李珠金.家禽无抗养殖中饲用乳酸菌应用研究进展[J].中国家禽,2018,40(11):41-46.
• [8]吴艳婕,贾文良.日粮中添加乳酸菌制剂对肉仔鸡生长性能的影响[J].现代畜牧科技,2015(7):37-38.
• [9]蒋磊,陈杰.乳酸菌制剂对肉鸡生长性能、盲肠菌群和免疫器官的影响[J].西昌学院学报（自然科学版）,2019,33(4):8-11.
• [10]鲁宇橦,陈群.乳酸菌在畜禽生产中应用的研究进展[J].黑龙江畜牧兽医,2019(3):44-46.
• [11]徐基利,许丽.不同乳酸菌及其添加水平对肉仔鸡生长性能、免疫机能和肠道结构的影响[J].动物营养学报,2011,23(11):1976-1983.
• [12]郭元晟.乳酸杆菌对肉鸡生产性能、免疫机能及肠道菌群的影响[D].呼和浩特:内蒙古农业大学,2011:29.
• [13]崔耀明,管军军,王金荣,等.益生乳酸菌对肉鸡骨骼发育的影响及作用机制研究进展[J].动物营养学报,2021,33(1):13-19.
• [14]Poorbaghi S L,Gheisari H,Dadras H,et al.Effects of simple and microencapsulated Lactobacillus acidophilus with or without inulin on the broiler meat quality infected by avian influenza virus(H9N2)[J].Probiotics Antimicro,2016,8(4):221-228.
• [15]张国华,岳小婧,于淇,等.饲粮添加黄芪和乳酸菌对青脚麻肉鸡生长性能、肠道微生物及抗氧化性能的影响[J].动物营养学报,2019,31(2):801-808.
• [16]周凌博,李继仁.饲料中添加乳酸菌固态制剂对肉鸡生长性能及免疫机能的影响[J].中国饲料,2019(5):62-64.
• [17]刘婷,李宗军,廖勇,等.饲喂乳酸菌和茶多酚对肉鸡生长及生理生化的影响[J].中国农学通报,2014,30(11):6-10.
• [18]Ahlberg S H,Joutsjoki V,Korhonen H J.Potential of Lacticacid bacteria in aflatoxin risk mitigation[J].Int J Food Microbiol,2015,207:87-102.
• [19]徐子涵,徐丽.不同剂型乳酸菌对雏鸡生长性能和小肠黏膜形态的影响[J].动物营养学报,2011,23(10):1812-1818.
• [20]黄崇波,郭志强,刁慧,等.百里香酚对肉兔生长性能、小肠黏膜形态结构和免疫器官的影响[J].中国畜牧杂志,2021,57(3):164-168.
• [21]付丽,赵巍,李立佳,等.乳酸菌培养物对肉鸡生长性能及免疫功能的影响[J].中国畜牧兽医,2015,42(9):2337-2344.
• [22]石峰,王涛,牛钟相.乳酸菌微生态制剂对肉鸡生产性能及免疫机能的影响[J].山东农业大学学报（自然科学版）,2011,42(1):79-83.
• [23]贾聪慧,杨彩梅,曾新福,等.丁酸梭菌对肉鸡生长性能、抗氧化能力、免疫功能和血清生化指标的影响[J].动物营养学报,2016,28(3):908-915.
• [24]冯云龙,王晶莹,于淼,等.丁酸梭菌对哺乳母猪繁殖性能、免疫和抗氧化功能的影响[J].中国畜牧杂志,2021,57(2):170-176.
• [25]张彩凤,齐广海,王晓翠,等.乳酸菌和酵母菌复合制剂及其与维吉尼亚霉素联合使用对肉仔鸡血清生化指标、抗氧化能力和免疫功能的影响[J].动物营养学报,2018,30(50):1893-1901.
• [26]尹艳军,郝延刚.冻干乳酸菌对肉鸡生长性能和免疫功能的影响[J].饲料工业,2015,36(10):36-40.
• [27]陈西风,周颖,刘维平.复合微生态制剂对肉仔鸡生长性能、肠道菌群和免疫功能的影响[J].畜牧与兽医,2019,51(7):55-59.
• [28]Mountzouris K C,Dalaka E,Palamidi I,et al.Evaluation of yeast dietary supplementation in broilers challenged or not with salmonella on growth performance,cecal microbiota composition and Salmonella in ceca,cloacae and carcass skin[J].Poult Sci,2015,94(10).2245-2255.