中国畜牧杂志

2021, v.57(07) 90-94

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miR-15b在荣昌猪不同体组织中的表达差异及其在C_2C_(12)细胞中的动态表达分析
Expression Characteristics of miR-15b in Different Tissues of Rongchang Pig and Its Dynamic Expression in C_2C_(12) Cells

龙熙;孙文阳;柴捷;王金勇;吴玲;张廷焕;
LONG Xi;SUN Wenyang;CHAI Jie;WANG Jinyong;WU Ling;ZHANG Tinghuan;Chongqing Academy of Animal Science;College of Animal Science and Technology, Gansu Agricultural University;Suijiang County of Animal Health Supervision Office;

摘要(Abstract):

为探究miR-15b在荣昌猪不同体组织中的表达差异及其在C_2C_(12)细胞不同发育时期的动态表达变化,本研究采用RT-qPCR方法检测miR-15b在荣昌猪心脏、肝脏、脾脏、肺、肾脏、胃、肌肉、胸腺和小肠组织及其在C_2C_(12)细胞增殖和分化过程中的表达水平。结果表明:miR-15b在荣昌猪各组织中均有表达,其中在心脏和胸腺中的表达量最高,在肝脏和肾脏中的表达量最低;miR-15b的表达量在C_2C_(12)细胞的整个增殖过程中均显著升高,在C_2C_(12)细胞的整个分化过程中先显著升高再显著降低。综上所述,miR-15b在荣昌猪各组织广泛表达,其表达量在C_2C_(12)细胞的增殖和早期分化中显著升高,推测miR-15b在C_2C_(12)细胞的增殖以及分化早期发挥重要功能。
In order to explore the expression characteristics of miR-15b in different tissues of pigs and different developmental stages of C_2C_(12) cells, RT-q PCR was used to detect the expression level of miR-15b in different tissues of Rongchang pigs and different stages of proliferation and differentiation of C_2C_(12) cells. The results showed that miR-15b is expressed in all tissues of Rongchang pigs, with higher expression level in heart and thymus and lower expression level in liver and kidney. The expression of miR-15b increased significantly during the proliferation process of C_2C_(12) cells and early differentiation of C_2C_(12) cells, but it's reduced in the late differentiation of C_2C_(12) cells. In general, miR-15b is widely expressed in various tissues of Rongchang pigs. The expression of miR-15b increased significantly in the proliferation and early differentiation of C_2C_(12) cells, which suggested that miR-15b may play an important role in the proliferation and early differentiation of C_2C_(12) cells.

关键词(KeyWords): miR-15b;荣昌猪;C_2C_(12)细胞;表达特征;细胞形态
miR-15b;Rongchang pig;C_2C_(12);Expression characteristics;Cell morphology

Abstract:

Keywords:

基金项目(Foundation): 重庆市绩效激励引导专项项目(19540);重庆市绩效激励引导专项项目(19536);; 国家自然科学基金项目(31771376)

作者(Author): 龙熙;孙文阳;柴捷;王金勇;吴玲;张廷焕;
LONG Xi;SUN Wenyang;CHAI Jie;WANG Jinyong;WU Ling;ZHANG Tinghuan;Chongqing Academy of Animal Science;College of Animal Science and Technology, Gansu Agricultural University;Suijiang County of Animal Health Supervision Office;

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DOI: 10.19556/j.0258-7033.20200828-08

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