中国畜牧杂志

2013, v.49(11) 92-96

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实时荧光定量PCR内参基因的选择
The Selection of Reference Gene in Real-Time Quantitative Reverse Transcription PCR

董恩妮;梁青;李利;王林杰;仲涛;王永;张红平;
DONG En-ni1,LIANG Qing1,LI Li1,WANG Lin-jie1,ZHONG Tao1,WANG Yong2,ZHANG Hong-ping1*(1.Institute of Animal Genetics and Breeding,Sichuan Agricultural University,Sichuan Ya′an 625014,China; 2.Southwest University for Nationalities,Sichuan Chengdu 610225,China)

摘要(Abstract):

实时荧光定量PCR(qRT-PCR)作为一种检测mRNA的敏感方法已被广泛应用于基因的表达分析。在利用qRT-PCR技术检测目的基因的表达水平时,为了减少检测样本在RNA产量、质量和反转录效率上可能存在的差异,需要选择合适的稳定内参基因作为校正标准,但是迄今为止尚未找到在所有条件下均可稳定表达的内参基因。近年来出现了筛选稳定性好的内参基因的新方法,如使用GeNorm软件、基因芯片数据、EST数据库。本文就qRT-PCR技术中内参基因的意义、参考基因在物种及组织上的表达特异性、内参基因选择方法进行了综述。
As a sensitive method for detection mRNA abundance,the quantitative real-time PCR(qRT-PCR) has been widely used to analyze gene expression.In order to reduce the possible differences in RNA quality and reverse transcription efficiency,it is necessary and important to choose suitable and stable reference genes as calibration standard in qRT-PCR.So far,no single gene which can act as a universal reference gene was reported.However,new methods to select stabilized reference genes have been discovered,such as the use of GeNorm software,gene-chip expression data and public deposited EST data.The present review will discuss about the selection of reference genes in qRT-PCR.

关键词(KeyWords): 实时荧光定量PCR;内参基因;稳定性
qRT-PCR;reference gene;stability

Abstract:

Keywords:

基金项目(Foundation): 四川省科技支撑计划(2011NZ0003、2011NZ0099-36)

作者(Authors): 董恩妮;梁青;李利;王林杰;仲涛;王永;张红平;
DONG En-ni1,LIANG Qing1,LI Li1,WANG Lin-jie1,ZHONG Tao1,WANG Yong2,ZHANG Hong-ping1*(1.Institute of Animal Genetics and Breeding,Sichuan Agricultural University,Sichuan Ya′an 625014,China; 2.Southwest University for Nationalities,Sichuan Chengdu 610225,China)

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